For each position where the seed matchs, a "priming score" is calculated taking into account data from Stadhouders et al. The algorithm starts seeking perfect matches of a sub-sequence (seed) in terminal position (3') of the primer against the whole target sequence and its complementary sequence. The calculations are based on data from Stadhouders et al. New match prediction algorithm that is compatible with dCAPS and authorizes mismatches all along the primer (including 3' end).New functionality for designing CAPS (Konieczny & Ausubel) et dCAPS (Neff et al.) genotyping strategies. (Menu : 'Extra > dCAPS').The graphic representation has been enriched with GC and complexity content for helping in quickly identify problematic/interesting regions in target sequence/amplicons.New functions have been added like CAPS/dCAPS design (for SNP genotyping).Many algorithms have been rethought in regard to new insights and real experimental designs in the field of PCR.AmplifX has been deeply modified and rewritten for 64 bit support.Add an export button in dCAPS results (to export all results as tab or csv file.).Improvement of primer suggestion in dCAPS design with very degenerated enzyme sites.Fix crash when a shared primer list is closed by the user with write rights and the read-only user is informed he can re open it.its start position is before the first nucleotide of the target) Fix crash when a primer matches with a "floatting" end on the target (i.e.Improvement of copy-paste from different binary sources (MacVector.Correction of AmplifX home page URL in the "About AmplifX" window and in the feedback.Fix display of accented characters in feedback forms and alert of new update.Improves behavior of adding primers to a new unsaved primer list.
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